ANTI-VILLUS CRYPT. CLONE E5D10 (C15-1885-289)

General description

Neuropilin-1 (UniProt: O14786; also known as Vascular endothelial cell growth factor 165 receptor, CD304) is encoded by the NRP1 (also known as NRP, VEGF165R) gene (Gene ID: 8829) in human. Neuropilins are highly conserved, non-tyrosine kinase, single-transmembrane glycoproteins that serve as co-receptors for various molecules, including semaphorins and vascular endothelial growth factors (VEGF). Neuropilin-1 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 22-856), a transmembrane domain (aa 857-879), and a cytoplasmic domain (aa 880-923). It acts as a receptor involved in the development of the cardiovascular system, angiogenesis, and formation of certain neuronal circuits. It is also reported to mediate the chemorepulsant activity of semaphorins. Neuropilin-1 has four well conserved domains: CUB (a1/a2; aa 27-141 and 147-265) domain, F5/8 (b1/b2; aa 275-424 and 431-583) domain, MAM domain (aa 645-811), and another domain that contains a transmembrane and a short cytoplasmic region. CUB domains mediate binding to semaphorins, while the tandem F5/8 domains are responsible for VEGF and heparin binding and recognizes a C-end role (CendR) motif R/KXXR/K on its ligands. The presence of the CUB (a1a2) domain is reported to enhance VEGF165 binding to F5/8 (b1b2) domain. The MAM domain is reported to mediate semaphorin signaling to Plexin-A. Neuropilin-1 has been recognized as an additional cellular mediator that facilitates the entry of SARS-CoV-2 into host cells. It binds to furin-cleaved spike protein and significantly potentiates SARS-CoV-2 infectivity. This effect can be blocked by monoclonal antibodies against the extracellular F5/8 (b1/b2) domain of Neuropilin-1 or with selective Neuropilin antagonists that bind to CendR binding b1 pocket. (Ref.: Kyrou, I., et al. (2021). Signal Trans. Targeted Ther. 6; Article number: 21; Daly, JL., et al. (2020). Science. 370(6518); 861-865; Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518); 856-860; Mamluk, R., et al. (2002). J. Biol. Chem. 277(27); 24818-24825).

Specificity

Clone mAb1 is a mouse monoclonal antibody that detects Neuropilin-1. It targets an epitope within the b1b2 domain in the extracellular region.

Immunogen

His-tagged recombinant fragment corresponding to 311 amino acids from the b1b2 region within the extracellular domain of human Neuropilin-1.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in PC-3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neuropilin-1 in PC-3 cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Neuropilin-1 in Immunofluorescence applications (Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518):856-860; Daly, J.L., et al. (2020). Science. 370(6518):861-865).

ELISA Analysis: A representative lot detected Neuropilin-1 in ELISA applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Immunocytochemistry Analysis: A representative lot detected Neuropilin-1 in Immunocytochemistry applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Neuropilin-1, ECD b1b2, clone mAb1, Cat. No. MABS2301, is a mouse monoclonal antibody that detects Neuropilin-1 (NRP1) and is tested for use in ELISA, Immunocytochemistry, and Immunofluorescence.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.