ANTI-VILLUS CRYPT. CLONE E5D10 (C005B-448809)

General description

C-type lectin domain family 18 member A (UniProt: A5D8T8; also known as Mannose receptor-like protein 2) is encoded by the CLEC18A (also known as MRLP2) gene (Gene ID: 2348174) in human. CLEC18 is a novel secretory lectin that is expressed in all cells but is abundantly expressed in hepatocytes and peripheral blood cells. It is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form (aa 27-446). Its C-type lectin domain is localized in amino acids 306-433. CLEC18 is mainly localized in the endoplasmic reticulum, Golgi apparatus, and endosome. Although it is expressed in normal hepatocytes, it is absent in hepatocellular carcinoma. It binds polysaccharides in a Ca2+-independent manner and shows preferential binding to fucoidal, β-glucans and galactans. It also serves as a co-receptor for TLR3 and contributes to the differential immune responses to poly(I:C) and H5N1 influenza A virus infection. Its levels are shown to be up-regulated upon differentiation of monocytes into macrophages and dendritic cells. Significantly higher levels of CLEC18A are observed subjects with hepatitis C infection and this increased level is associated with increased cryoglobulin levels, which may be related to the occurrence of mixed cryobulinemia (MC) syndrome. (Ref.: Huang, YL., et al. (2021). Nat. Commun. 4;229; Liao, TL., et al. (2018). Sci. Rep. 8; Article 17287; Huang, YL., et al. (2015). J. Biol. Chem. 290(35); 21252-21263).

Specificity

Clone 3F10F is a mouse monoclonal antibody that detects C-type lectin domain family 18 member A (CLEC18A).

Immunogen

His/Sumo-tagged full-length recombinant mature C-type lectin domain family 18 member A (CLEC18A) (without the signal peptide).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Human CLEC18A protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human CLEC18A protein.

Tested Applications

ELISA Analysis: A representative lot detected CLEC18A in ELISA applications (Liao, T-L., et al. (2018). Sci Rep. 8(1); 17287).

Flow Cytometry Analysis: A representative lot detected CLEC18A in Flow Cytometry applications (Liao, T-L., et al. (2018). Sci Rep. 8(1); 17287).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CLEC18A, clone 3F10F, Cat. No. MABS2280, is a mouse monoclonal antibody that detects CLEC18A and is tested for use in ELISA, Flow Cytometry, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.