Specificity

Vimentin (58kD). Reacts in immunocytochemical assays with all mesenchymal cells (fibroblasts, Scwann cells, endothelial cells, smooth muscle cells, etc.).

Application

Immunohistochemistry: 1:500-1:2,000 on paraffin embedded sections (ABC peroxidase) fixed with alcohol (e.g. Carnoy) with a 2" pronase digestion. Aldehyde fixation is not recommended.

Immunocytochemistry: 1:100-1:200 cells in vitro (IFA) fixed in ice cold absolute methanol or acetone.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Vimentin Antibody, clone 43BE8 is validated for use in IC, IH(P) for the detection of Vimentin.

Physical form

Liquid

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.