Anti-VPAC2 Antibody (C15-1315-973)

General description

Vasoactive intestinal polypeptide receptor 2 (VPAC2), also known as PACAP type III receptor or PACAP-R3 for short, is a class B G-protein-coupled receptor. Three distinct PACAP receptors have been identified. Of the three, only PACAP-3 has been found expressed in Sartoli cells of the testis and is coupled to G and adenylyl cyclase, thus having a potential influence on the production of cAMP. In the immune system, PACAP-R3 is expressed on stimulated CD4 T cells, and binding of T cell-derived VIP to-PACAP-R3 induces a shift toward the Th2 pathway. In addition, PACAP-R3 is an essential regulator of the circadian pacemaker of the hypothalamic suprachiasmatic nuclei (SCN).

Specificity

Other homologies: mouse (84% sequence homology).

This antibody recognizes VCAP2 (PACAP-R3) at the extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to the extracellular domain of human VPAC2 (PACAP-R3).

Application

Anti-VPAC2 Antibody is an antibody against VPAC2 for use in WB, IH(P).

Immunohistochemistry Analysis: 1:400 dilution from a previous lot detected VPAC2 in normal human cerebral cortex and T cells inside the mantle zone of normal human tonsil tissues.

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected VPAC2 on 10 µg of Jurkat cell lysate.

Target description

~52 kDa observed. An uncharacterized band appears at ~39 kDa in some lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): pig (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_003373. UniProt accession no.: P41587. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... VIPR2(7434). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.