Anti-WAC Antibody, CT (C15-1317-171)

General description

WAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at ′Lys-120′ (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the C-terminus of the WAC protein.

Immunogen

Recombinant protein corresponding to human WAC.

Application

Anti-WAC, C-terminus detects levels of WAC proteins & has been published & validated for use in wb, IP & ChIP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blotting Analysis: 1 µg/mL of this antibody from a representative lot detected WAC, C-terminus in 10 µg of HEK293 cell lysate.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

Westerm Blotting Analysis: A representative lot from an independent laboratory detected WAC in 293T cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in 293T cell lysates transfected with siWAC (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, C-terminus in 10 µg of K562 cell lysate.

Target description

~80 kDa observed. An uncharacterized band may be observed at ~95 kDa and ~120 kDa in some cell lysates.

Physical form

Affinity purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
K562 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. technique(s): ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_057712. UniProt accession no.: Q9BTA9. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... WAC(51322). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.