Anti-WAS protein family homolog 1 (CT) Antibody (C15-1317-927)

General description

Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) is a novel protein belonging to the WASP family, a group of nucleation promoting factors (NFPs) that activate a critical component in endocytosis, Arp 2/3. WASH contains three specific domains: a C-terminal VCA domain, a proline-rich domain, and an N terminal region containing two WASH homolog regions, WHD1 and WHD2. Although WASH has yet to be completely characterized, it is known to function as an NPF at the endosomal surface, where it is involved in the recruitment and subsequent activation of Arp2/3 in order to initiate actin polymerization. Therefore, WASH has a crucial role in endosomal sorting as a mediator of tubule fission resulting from Arp2/3 activation.

Specificity

This antibody recognizes WAS protein family homolog 1 at the C-terminus.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to human WAS protein family homolog 1 at the C-terminus.

Application

Anti-WAS protein family homolog 1 (C-terminus) Antibody detects level of WAS protein family homolog 1 (C-terminus) & has been published & validated for use in WB.

Research Category
Signaling

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected WAS protein family homolog 1 on 10 µg of HeLa cell lysate.

Target description

~ 60 kDa observed. The calculated molecular weight is 50 kDa

Physical form

Format: Purified

Protein G

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human. technique(s): western blot: suitable. isotype: IgG. NCBI accession no.: NP_878908. UniProt accession no.: A8K0Z3. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... WASH1(100287171). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.