Anti-WASH complex subunit 7 (SWIP) Antibody (C15-1318-079)

General description

WASH complex subunit 7 (SWIP) is a component of the WASH complex belonging to the WASH S7 family. The WASH complex is localized at early endosomal subdomains involved in the activation and recruitment the ARP 2/3 complex in order to stimulate actin polymerization and tubulin interaction. Through its interaction with FAM21, WASH plays a regulatory role in the retromer-mediated trafficking of vesicles. It is also involved in the tubule fission, components required in endosomal sorting processes as transport intermediates.

Immunogen

GST-tagged recombinant protein corresponding to human WASH complex subunit 7.

Application

Detect WASH complex subunit 7 (SWIP) using this Anti-WASH complex subunit 7 (SWIP) Antibody validated for use in IP.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Immunoprecipitation/Western Blot Analysis in HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7.

Immunoprecipitation/Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit 7 in 10 µg of HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7.

Target description

~135 kDa observed.
IgG heavy chain may be observed at approximately 50 kDa

Physical form

Format: Purified

Protein G Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. technique(s): immunoprecipitation (IP): suitable. NCBI accession no.: NP_056090.1. UniProt accession no.: Q2M389. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... WASHC4(23325). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.