ANTI-XPA (C-TERMINAL) ANTIBODY PRODUCED IN RABBIT IGG FRACTION OF ANTISERUM

General description

Xeroderma pigmentosum group A-complementing protein (XPA) or DNA repair protein complementing XP-A cells human excision repair protein. XPA encodes a hydrophilic metalloprotein. It displays a C4 zinc finger motif, a central globular domain and has disordered regions in the N and C-terminus. The gene XPA is localized in human chromosome 9q22.33.

Specificity

Anti-XPA (C-terminal) specifically recognizes human XPA.

Immunogen

synthetic peptide corresponding to amino acids 257-273 of human XPA, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences by one amino acid.

Application

Anti-XPA (C-terminal) antibody produced in rabbit has been used in immunolabeling and immunoblotting.

Biochem/physiol Actions

Xeroderma pigmentosum group A-complementing protein (XPA) interacts with nucleotide excision repair (NER) subunits, such as replication protein A (RPA), excision repair complementing 1 protein (ERCC1), and transcription factor II (TFIIH). However, it is widely accepted that the Xeroderma pigmentosum group C-complementing protein (XPC)-human Rad23 homolog (hHR23B) complex recognizes the DNA damage-induced helical distortion. After this, the transcription factor IIH (TFIIH), XPA (possibly in its homodimeric form), and replication protein A (RPA) arrive sequentially at the site of damage. XPA interacts directly with DNA via the zinc finger motif. RPA coordinates with XPA in the positioning in the nucleotide excision repair (NER) bubble. Defects in the excision repair leads to photosensitivity syndrome called xeroderma pigmentosum (XP).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

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