ANTI-ZAP-70 ANTIBODY CLONE 2F3.2 FITC CONJUGATE

General description

Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals

Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC)

Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254

ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.

Specificity

Expected to cross-react with rat.

Recognizes human ZAP-70.

Immunogen

GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 correspon-ding to amino acids 1-254. Clone 2F3.2.

Application

Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate detects level of ZAP-70 & has been published & validated for use in FC, IC & WB.

Immunocytochemistry:
5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.

Research Category
Signaling

Research Sub Category
Immunological Signaling

Quality

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.

Target description

70 kDa

Physical form

Mouse monoclonal FITC-conjugated IgG2a in buffer containing PBS containing 0.05% sodium azide.

Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.

Protein G Purified

Storage and Stability

Stable for 6 months at 2-8°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing cap.

Analysis Note

Control
Western Blot: Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.