ANTI-ZCCHC3 CLONE 1E10 ZOOMAB RABBIT MONOCLONAL (C005B-262228)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1E10 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Zinc finger CCHC domain-containing protein 3 (ZCCHC3). It targets an epitope within 21 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Zinc finger CCHC domain-containing protein 3 (ZCCHC3).

Application

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZCCHC3 in HepG2 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZCCHC3 in mouse placenta tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ZCCHC3 in human placenta tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZCCHC3 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Zinc finger CCHC domain-containing protein 3 (UniProt: Q9NUD5; also known as ZCCHC3) is encoded by the ZCCHC3 (also known as C20orf99) gene (Gene ID: 85364) in human. ZCCHC3, a CCHC-type zinc-finger protein, serves as a nucleic acid-binding protein involved in innate immune response to DNA and RNA viruses. It binds DNA and RNA in the cytoplasm and acts by promoting recognition of viral nucleic acids by virus sensors, such as DDX58/RIG-I, IFIH1/MDA5 and cyclic GMP-AMP synthase (cGAS). Also acts as a co-sensor for recognition of double-stranded DNA (dsDNA) by cGAS. Hence, it plays a role in innate immune response to cytosolic dsDNA and DNA virus. It is shown to directly bind to dsDNA and enhance the binding of cGAS to dsDNA. ZCCHC3-deficiency is reported to inhibit dsDNA- and DNA virus-triggered induction of downstream effector genes and severely impair the induction of serum cytokines. Mice deficient in ZCCHC3 are shown to be more susceptible to lethal herpes simplex virus type 1 or vaccinia virus infection. Two isoforms of ZZCHC3 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lian, H., et al. (2018). Nat. Commun. 9; Article number: 3349).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.