Anti-ZEB1 Antibody (C15-1317-207)

General description

Zinc finger E-box-binding homeobox 1 (ZEB1), also known as Transcription factor 8 (TCF8), Negative regulator of IL2, and NIL-2-A zinc finger protein acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 has also been implicated in regulating the expression of ATP1A1, E-cadherin, BCL6, and RCOR1 genes. ZEB1 regulates neurogenesis and neuronal differentiation, but is also implicated in tumor formation. ZEB1 is expressed in heart and skeletal muscle. Mutations in ZEB1 have been associated with corneal dystrophy.

Immunogen

KLH-conjugated linear peptide corresponding to human ZEB1 .

Application

Detect ZEB1 using this rabbit polyclonal antibody, Anti-ZEB1 Antibody validated for use in western blotting, ICC & Immunofluorescence.

Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected ZEB1 in HeLa cells.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ZEB1 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ZEB1 in 15 µg of HeLa cell lysate.

Target description

~130 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes two isoforms at ~124 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable. UniProt accession no.: P37275. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ZEB1(6935). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.