Anti-ZEB2 Antibody (C15-1318-050)

General description

Zinc finger E-box-binding homeobox 2 (ZEB2), also known as Smad-interacting protein 1 (SIP1, SMADIP1) and Zinc finger homeobox protein 1b (ZFHX1B, ZFX1B), is a transcriptional inhibitor that binds to DNA sequence 5′-CACCT-3′ in different promoters and interacts with activated SMADs. ZEB2 has been shown to repress the transcription of the E-cadherin gene and induce epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing in vitro. It is suggested that ZEB2 contributes to tumor progression since it’s been demonstrated to protect cells from DNA damage-induced apoptosis. Recent studies have shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, implying that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Mowat-Wilson syndrome (MWIS), also known as Hirschsprung disease-mental syndrome.

Immunogen

KLH-conjugated linear peptide corresponding to human ZEB2.

Application

Detect ZEB2 using this rabbit polyclonal antibody, Anti-ZEB2 Antibody validated for use in western blotting, ICC & Immunofluorescence.

Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected ZEB2 in Jurkat cells.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ZEB2 in Jurkat cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ZEB2 in 15 µg of Jurkat cell lysate.

Target description

~200 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~134 kDa and ~136 kDa The calculated molecular weight of this protein is 136 kDa, but can be observed at ~200 kDa due to post-translational modification.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable. UniProt accession no.: O60315. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ZEB2(9839). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.