ANTI-ZIKA VIRUS NS1 PROTEIN. CL 680 (C005B-450478)

Specificity

Clone 680 is a mouse monoclonal antibody that detects Zika virus NS1 protein.

Immunogen

His-tagged recombinant Zika Virus NS1 protein expressed in mammalian cells.

Application

Quality Control TestingEvaluated by ELISA with recombinant Zika virus NS1 antigen.ELISA Analysis: 1 μg/mL of this antibody detected recombinant Zika Virus NS1 protein.

Anti-Zika Virus NS1 protein, clone 680, Cat. No. MABF2186, is a mouse monoclonal antibody that detects Zika Virus NS1 protein and is tested in ELISA.

Target description

Genome polyprotein (UniProt: Q32ZE1) following its synthesis is cleaved into 13 different proteins in Zika virus. Zika virus is a member of the Flaviviridae family that contains a single positive sense RNA genome. Following the fusion of virus membrane with host endosomal membrane, RNA genome is released into the cytoplasm. It is initially translated as a single polyprotein, which is post-translationally cleaved into three structural proteins (C, PrM or M, and E) and seven nonstructural (NS) proteins. Six of the NS proteins (NS2A to NS5) are shown to form a replication complex on the cytoplasmic side of the endoplasmic reticulum membrane. The glycosylated NS1 that is reported to associates with lipids, forms a homodimer inside the cells and is shown to be essential for viral replication and late in infection. It is also secreted into the extracellular space as a hexameric lipoprotein particle and plays a role in immune evasion and pathogenesis via its interaction with components from both the innate and adaptive immune systems. NS1 is also a major antigenic marker for viral infection. NS2A functions in virion assembly and antagonizes the host immune response. The non-structural protein 2B is essential for the serine protease function of NS3. The serine protease NS3 has three different enzymatic activities - serine protease, NTPase and RNA helicase. The non-structural protein 4A of virus regulates the ATPase activity of the NS3 helicase while the non-structural protein 4B inhibits interferon-induced host STAT1 phosphorylation and its nuclear translocation. (Ref.: Song, H. et al. (2016). Nat. Struct. Mol. Biol. 23(5); 456-458; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.