ANTI-ZO-1 CLONE 4E14 ZOOMAB RB MONO (C15-1670-072)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 4E14 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Tight junction protein ZO-1.

Immunogen

GST/His-tagged recombinant fragment corresponding to 145 amino acids from the N-terminal half of human Tight junction protein ZO-1 (ZO1).

Application

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZO-1 in A549 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in HepG2, MCF-7, and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ZO-1 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZO-1 in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ZO-1, clone 4E14 ZooMAb^®, Cat. No. ZRB1761, is a recombinant Rabbit monoclonal antibody that specifically targets ZO-1 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Tight junction protein ZO-1 (UniProt: Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 (also known as ZO1) gene (Gene ID: 7082) in human. TJP1, TJP2, and TJP3 (ZO-1, ZO-2, and ZO-3) are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. ZO-1 is a homodimeric peripheral membrane protein that can also form heterodimers with TJP2 and TJP2 via its PDZ2 domain (aa 296-264). ZO-1 is reported to be essential for lumenogenesis and for efficient epithelial polarization and barrier formation. It also plays a role in podosome formation and associated functions, thus regulating cell adhesion and matrix remodeling. At podosome sites, it is predominantly localized in the ring structure surrounding the actin core. Two isoforms of ZO-1 have been described. The long isoform (-isoform) is found in most epithelial junction and the short isoform that lacks amino acids 922-1001 is found both in epithelial cells and in epithelial junctions of renal glomeruli and Sertoli cells of seminiferous tubules. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.