Anti-Zscan4 Antibody (C15-1316-157)

General description

Zinc finger and SCAN domain containing protein 4, also called Zscan4, is an embryonic stem (ES) cell-specific transcription factor required to regulate pluripotency. Zscan4 binds and regulates telomere elongation in order to maintain genomic stability and acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) in undifferentiated ES cells.

Immunogen

KLH-conjugated linear peptide corresponding to mouse Zscan4.

Application

Anti-Zscan4 Antibody is a highly specific rabbit polyclonal antibody, that targets Zinc Finger Protein & has been tested in western blotting, Immunofluorescence & IP.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Zscan4 in mouse iPS and R1 Embyonic Stem cells. Data courtesy of the Melanie Welham lab at The University of Bath, UK
Immunoprecipitation Analysis: A 1:4,000 dilution from a representative lot detected Zscan4 in cytoplasmic and nuclear extracts in IP experiments conducted by the Melanie Welham lab at The University of Bath, UK. (Data not shown)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in mouse Embryonic Stem cell lysates.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Zscan4 in 10 µg of Embryonic Stem cell lysates.

Target description

~65 kDa observed. The calculated molecular weight of this protein is 57 kDa An uncharacterized band may be observed at ~50 kDa in some cell lysates.ZSCAN4 bands may be observed at both ~72 kDa and ~40 kDa when preparing cell lysates with SDS buffer. Additionally, a ZSCAN4 doublet may be observed at ~72 kDa when using cytosolic or nuclear extracts.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Embryonic Stem Cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. technique(s): immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_001013787. UniProt accession no.: Q80VJ6. shipped in: wet ice. target post-translational modification: unmodified. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.