Anti-Zyxin (C15-1316-627)

General description

Zyxin (UniProt: Q15942; also known as Zyxin-2) is encoded by the ZYX gene (Gene ID: 7791) in human. Zyxin is an evolutionarily conserved LIM domain protein that acts as an adhesion plaque protein concentrated at sites of cell adhesion and is widely expressed. It has been implicated in cell adhesion, cell motility, and signaling. Zyxin is reported to associate with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family of cytoskeletal regulators and plays a role in cytoskeletal dynamics and signaling. Zyxin contains three LIM zinc binding domains (aa 384-443; 444-503; and 504-570), which act as a negative regulator of Zyxin-VASP complexes. Also, it contains four proline rich ActA repeats (aa 64-77; 94-108; 115-121; and 127-137) that are known to interact directly with Ena/VASP proteins. Loss of Zyxin results in inability to thicken stress fibers in response to mechanical stimuli. (Ref.: Hoffman LM et al (2003). Mol. Cell Biol. 23(1); 70-79; Moody JD et al. (2009). Biochem. Biophys. Res. Commun. 378(3), 625-628; Yoshigi et al (2005). J. Cell Biol. 171(2), 209-215.).

Specificity

This rabbit polyclonal antibody detects Zyxin in human and murine cells. It targets an epitope within 20 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Zyxin.

Application

Anti-Zyxin Antibody, Cat. No. ABC1387, is a rabbit polyclonal antibody that detects Zyxin in human and murine cells and has been tested for use in Immunocytochemistry and Western Blotting.

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Zyxin in 10 µg of NIH/3T3 cell lysate.

Immunocytochemistry Analysis: A representative lot detected Zyxin in Immunocytochemistry applications (Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82).

Western Blotting Analysis: A representative lot detected Zyxin in Western Blotting applications(Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82).

Immunocytochemistry Analysis A 1:600 dilution from a representative lot detected Zyxin in mouse fibroblasts (Courtesy of Dr. Laura Hoffman at Huntsman Cancer Institute, University of Utah).

Western Blotting Analysis: A representative lot detected Zyxin in Western Blotting applications (Moody, J.D., et. al. (2009). Biochem Biophys Res Commun. 378(3):625-8).

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Zyxin in mouse fibroblast, HeLa, and U2OS cell lysates (Courtesy of Dr. Laura Hoffman at Huntsman Cancer Institute, University of Utah).

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Zyxin in 10 µg of A431 cell lysate.

Target description

~75 kDa observed; 61.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human. species reactivity (predicted by homology): monkey (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. L. technique(s): immunocytochemistry: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_003452. UniProt accession no.: Q15942. target post-translational modification: unmodified. Gene Information: human ... ZYX(7791). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.