Antibiotic Assay media are used in the performance of antibiotic assays. Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays (1). Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay (2). This media is prepared according to USP (3) and by FDA (4). This medium is as per specification of Krishbaum and Arett (5). Peptic digest of animal tissue (Peptone), yeast extract and beef extract provides nutrients and growth factor. Dextrose provides the energy source and sodium chloride maintains the osmotic equilibrium of the medium. Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar precooled to 40-45°C and spread evenly over the surface of solidified base agar. Storage and Shelf-life: Store below 30°C in tightly closed container and use freshly prepared medium . Use before expiry date on the label. References: 1. Grove and Randall, 1955, Assay Methods of Antibiotics Medical Encyclopedia, Inc, New York. 2. Schmidt and Moyer, 1944; J. Bact, 47:199. 3. United States Pharmacopoeia 2009. US Pharmacopoeial Convention Inc, Rockville, MD. 4. Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983. Title 21, part 436, Subpart D, Washington, D.C. U.S Government printing office, paragraphs 436, 100-436, 106 pg 242-259 (April 1). 5.Krishbaum A and Areet B, 1967, J. Pharm Sci, 56: 512.
- UPC:
- 41106200
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- M101-5KG
