Antifoam 204 (C09-0738-388)

Product DescriptionAntifoam function as an effective foam suppressor. Antifoam 204 contains 100% active components and is a mixture of organic non-silicone polypropylene-based polyether dispersions. It does not contain mineral oil. Antifoam 204 can itself be considered a surfactant, but it contains no other surfactants. This product is synthetic and not derived from animal or plant sources. Antifoam 204 can be sterilized repeatedly. The flow properties of Antifoam 204 are such that it can be pumped to a fermenter on an as-needed basis. For use in microbiological media, Alddin recommends a starting concentration of between 0.005% and 0.01%.Antifoams are supplied as two basic types of composition or mixtures of the two. Organic antifoams are of synthetic origin. Silicone-based antifoams are generally considered to be siloxane polymers and are also synthetic. Our suppliers generally consider the exact composition to be proprietary.Products  are listed as Molecular Biology Grade and have been tested for use in bacterial fermentation. No significant inhibition is found when E. coli or B. subtilis is grown in Terrific Broth supplemented with effective concentrations of antifoams. These products have individual data sheets available upon request.Procedure Antifoams should be added prior to the point where foaming occurs. Alternatively, antifoam can be used as supplied, by wiping on filling nozzles, rims of processing vats, or on a screen suspended above the foaming system. One of the most common applications for antifoams is in microbiological fermentation.Since one cannot predict the effectiveness of an antifoam for a particular application, antifoams should also be tested to ensure adequate defoaming effectiveness under representative culture conditions for each microorganism. The variables of medium composition, temperature, pH, mixing, and aeration anticipated for the final experimental or fermentation conditions should be used. If the antifoam is not effective under these test conditions either a higher amount of antifoam can be added or a different type of antifoam can be tested. ApplicationsAntifoam 204 has been used:to suppress bubbling in thylakoid solutions during intramembrane isoprene quantificationas a component of fed-batch continuous culture fermentation mediumto suppress severe foaming in fermenterThe application of antifoam silicones in the microbiological fermentation technique.Foam formation and the subsequent cell damage/ losses in the foam layer were found to be the major problems affecting cell growth and monoclonal antibody (MAb) production in stirred and sparged bioreactors for both serum-supplemented and serum-free media. Surfactants in the culture media had a profound effect on cell growth by changing both the properties of bubbles and the qualities of foam formed.Addition of high concentrations of silicone antifoam to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component.The mechanism of antifoaming in a nonionic Tritonä X-100 surfactant solution with silicone polyethers, the so-called "cloud point antifoams," was investigated. The cloud point (CP) studies showed that the CP of a Triton X-100/silicone polyether mixed system is between the CP of the foaming and the antifoam surfactants, due to mixed micelle formation.Supercritical carbon dioxide was used for the direct extraction of drugs from plasma prior to analysis. The supercritical fluid was directly passed through plasma samples spiked with either a neutral (flavone) or an acidic (ketorolac) drug. The addition of an antifoam agent to the plasma prior to extraction was required to avoid restrictor plugging caused by denaturation of theplasma proteins by the supercritical fluid.Other NotesContains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly. Specification: mixture of organic polyether dispersions Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/A489541-SCI_49eacadcb997ae6c516adf18bd58c046.pdf