Antifoam A concentrate (C15-1315-357)

General description

Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.

Application

Antifoam A concentrate has been used:

• in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.
• to prevent foam formation during the aerobic treatment of sludge.
• to study the impact of surface tension on bioaerosol generation during bubbling.

Features and Benefits

• Molecular biology grade and tested for use in bacterial fermentation.
• Extremely effective foam suppressor for aqueous and non-aqueous systems.
• Made of 100% active silicone polymer, free of emulsifiers.
• Typically, effective at 1-100 ppm.
• Product will be stable in the pH range of 5 to 9.
• Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.

Components

Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .

Preparation Note

Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.

Quality Level: 200. form: emulsion (aqueous). technique(s): cell culture | hybridoma: suitable, microbiological culture: suitable. density: 0.97 . g/mL . at 25 . °C. application(s): microbiology. storage temp.: room temp. suitability: (Mammalian (suspension)), bacteria (fermentation). Pictograms: GHS09. Signal Word: Warning. Hazard Statements: H410. Precautionary Statements: P273 - P391 - P501. Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): 213.8 °F - closed cup. Flash Point(C): > 101 °C - closed cup. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.