Antifoam C Emulsion (C15-1315-437)

General description

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.

Application

Antifoam C emulsion has been used:

• as a supplement in RO-H₂O and a chemically defined culture medium to determine volumetric mass transfer coefficient (k_La) of mammalian cell culture
• as a component of feed medium,
• as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
• as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions

Features and Benefits

• Minimizes compatibility issues with biological systems
• Highly effective defoamer at low concentrations
• Highly effective and versatile silicone defoamer

Other Notes

A 30% aqueous emulsion of polydimethylsiloxane concentrate.

Quality Level: 200. form: emulsion. contains: emulsifier. technique(s): cell culture | hybridoma: suitable, microbiological culture: suitable. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H319 - H335. Precautionary Statements: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.