Antifoam SE-15 (C15-1315-477)

General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used:

• in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
• to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
• as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
• as a component of autoinduction expression culture.
• as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Features and Benefits

• Controls foaming in microbial culture flasks or fermentations
• Non-toxic to the culture medium
• Can be pumped to fermenter on an as-needed basis

Components

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

Quality Level: 200. form: emulsion (aqueous). concentration: 10% (active silicon). technique(s): cell culture | hybridoma: suitable. solubility: H₂O: soluble (effective hot or cold.). density: 1.0 . g/mL . at 25 . °C. application(s): microbiology. storage temp.: room temp. suitability: (Mammalian (suspension)), bacteria (fermentation).