Aphidicolin, Ready Made Solution (C15-1237-114)

General description

Aphidicolin is a tetracyclic diterpene with antiviral and antimitotic properties used for cell cycle synchronization in various cell lines.

Application

Aphidicolin, from Nigrospora sphaerica, was used for cell cycle synchronization of tobacco BY-2 cells. It is used to block the entry of cells into S-phase.

Biochem/physiol Actions

Aphidicolin specifically inhibits DNA polymerase α that is responsible for DNA replication. It also inhibits α-like DNA polymerases of plants and yeasts but does not inhibit synthesis of RNA and proteins. Aphidicolin competes for the dCTP-specific binding site on DNA polymerase α specifically. Aphidicolin has antiviral and antimitotic properties. It blocks the cell cycle at S phase. It has been shown to induce apoptosis in HeLa cells.

Packaging

1 ml

Physical form

Aphidicolin Ready Made Solution supplied as a 1 mg/ml solution in DMSO.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Storage class (TRGS 510): Combustible liquids

Molecular Weight: 338.48. Empirical Formula: C₂₀H₃₄O₄. biological source: Nigrospora sphaerica. Quality Level: 200. vapor pressure: 0.55 hPa ( 20 °C). form: liquid. color: clear colorless. solubility: H₂O: miscible (completely). antibiotic activity spectrum: neoplastics, viruses. Mode of action: DNA synthesis | interferes, enzyme | inhibits. storage temp.: −. 20°C. InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1. InChI key: NOFOAYPPHIUXJR-APNQCZIXSA-N. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): 188.6 °F - closed cup. Flash Point(C): 87 °C - closed cup.