Apo-Direct TUNEL Assay Kit

General description

The CHEMICON APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.

Application

Research Category
Apoptosis & Cancer

The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.

Components

The following components are included with the APO-DIRECT Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.

Positive Control Cells,Brown cap

Negative Control Cells, Natural cap

Wash Buffer, Blue cap

Reaction Buffer, Green cap

TdT Enzyme, Yellow cap

F-dUTP, Orange cap

Rinsing Buffer, Red cap

PI/RNase Staining Buffer, Amber bottle

Storage and Stability

Precautions and Warnings:

1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.

3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.