General description

Ascentis Express 5 μm HILIC is a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core^® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell around and an overall particle size of ~5-microns. The bare silica stationary phase of Ascentis Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.

Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m²/g and an average pore size of 90Ã…. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m²/g range.

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

material: stainless steel column. Quality Level: 100. Agency: suitable for USP L3. product line: Ascentis^®.. feature: endcapped: no. manufacturer/tradename: Ascentis^®.. packaging: 1 ea of . parameter: 60 Â°C temp. range, 600 bar max. pressure (9000 psi). technique(s): HPLC: suitable, LC/MS: suitable. L Ã— I.D.: 15 . cm Ã— 2.1 . mm . surface area: 90 . m²/g . impurities: <. 5 . ppm metals. matrix: Fused-Core particle platform, superficially porous particle. matrix active group: silica phase. particle size: 5 . μ. m. pore size: 90 . Ã… pore size. operating pH: 1-8. application(s): food and beverages. separation technique: hydrophilic interaction (HILIC), normal phase.