Asparagine Nitrate Medium, 500G

Asparagine Nitrate Medium is formulated as per Subba Rao (3). Nitrogen transformation in soil results in the loss of molecular nitrogen. The conversion of nitrate and nitrite into molecular nitrogen or nitrous oxide through microbial processes is known as denitrification. Denitrification of bound nitrogen to gaseous nitrogen is mediated by numerous species of bacteria, which normally use oxygen as hydrogen acceptor (aerobic). These bacteria also posses the ability to use nitrate and nitrite in the place of oxygen as the hydrogen acceptor (anaerobically). Asparagine is source of organic nitrogen and is readily available for microbial energy and growth while the salts in the medium help for growth of microorganisms. Application: Used for the Isolation and Cultivation of Denitrifying Bacteria from Soil Samples Appearance: White to cream homogeneous free flowing powder Gelling: Firm, comparable with 1.5% Agar gel Color and Clarity of prepared medium: Light amber colored clear to slightly opalescent gel forms in Petri plates Cultural Response: Cultural characteristics observed after an incubation at 25-30 deg C for upto 7 days