DescriptionAspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells andLactococcus lactisstrain cultured in lysogeny broth (LB) medium.Suitability: The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.Principle: Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric). Specification: sufficient for 100colorimetricorfluorometrictests Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/A486313-SCI_bcebb6c021eb255ae7252f37e53d6a66.pdf
- UPC:
- 41141610
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 3.53 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- A486313-1kit
- Product Size:
- 1kit
