General description
Adenosine triphosphate (ATP) is a key molecule that stores energy within cells, and is considered as the universal energy currency for all living organisms. ATP has been used as a marker of viability, as living cells have high ATP content whereas dead cells lose their ability to produce ATP. ATP levels are therefore proportional to the amount of living cells in a sample.
The ATP Bioluminescence Kit provides a simple and quick assay to quantify ATP, and also to measure cell viability based on ATP content. This assay is less prone to artifacts than other viability assay methods. Intracellular ATP is released by permeabilization solution and measured using a bioluminescence assay, owing to the ability of luciferase to produce light in the presence of its substrate D-luciferin and ATP.
The kit has the following linear detection range:
- 0 - 60,000 cells (tested on TF-1 cells)
- 1nM-10μM ATP
Application
Quantifing ATP within a sample
Measure cell viability based on ATP content
Features and Benefits
Our economical ATP Bioluminescence Kit also saves your time and efforts:
- Highly sensitive, simple and high-throughput assay for measuring ATP in biological samples
- User friendly: No need to weigh or calculate needed amounts of reagents on your own
- Supportive calculator (Click here to download a calculator excel file): will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
- Quick instruction bench card - to assure your experimental success
- More experiments in one kit - contains sufficient reagents for 200 tests
- UPC:
- 41116012
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CS0012-1KT
