ATP-GAMMA-S SOLUTION

General description

Adenosine 5′-O-(3-thiotriphosphate), lithium salt, solution.

Application

ATP-γ-S has been used:

• to treat THP-1 macrophage cell lines for testing if endoplasmic reticulum (ER) stress causes the production of interleukin (IL)-1β
• in amide hydrogen exchange (HX) experiments coupled to mass spectrometry and study the cyclomarin A (CymA)-induced structural changes in N_Mtb-ClpC
• as a supplement in CMG-binding buffer for gel-based DNA unwinding assays

Biochem/physiol Actions

Adenosine 5′-O-(3-thiotriphosphate) (ATP-γ-S) is a substrate and inhibitor of ATP-dependent enzyme systems. It is hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. ATP-γ-S serves as a damage-associated molecular pattern molecule (DAMP) and NLR family pyrin domain containing 3 (NLRP3) inflammasome activator. ATP-γ-S may serve as a substrate for RNA-stimulated nucleotide hydrolysis and RNA unwinding activities of eukaryotic initiation factor-4A (eIF4A).

Quality

Contaminants: ≤10% ADP (HPLC)

Other Notes

For life science research only. Not for use in diagnostic procedures.