General description
Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.
Features and Benefits
- Strong absorption
- High fluorescence quantum yield
- High thermal and photo-stability
Other Notes
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Molecular Weight: 708.11. Empirical Formula: C35H34ClN3O11. product line: BioReagent. Quality Level: 100. Assay: ≥. 90% (HPLC), ≥. 90% (degree of coupling). form: powder. manufacturer/tradename: ATTO-TEC GmbH. suitability: suitable for fluorescence. detection method: fluorometric. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).- UPC:
- 51295001
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 72464-1MG-F