General description
illustra™ Autoseq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results.
illustra™ Autoseq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data.
llustra Autoseq™ G-50 consists of MicroSpin™ columns containing Sephadex® G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.
illustra™ Autoseq™ G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.
Features and Benefits
- For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing.
- Columns are prepacked with Sephadex® G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative.
- Columns that are ready to use enable sample application to collection of purified product in less than 4 min.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
AutoSeq is a trademark of Cytiva
MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
illustra is a trademark of Cytiva
- UPC:
- 41115717
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- GE27-5340-02
