General description

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

Application

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.

Avidin-Agarose from egg white has been used:

in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
in avidin bead assay for purification of labelled biotin
for capture of glycoproteins from murine cornea samples

Biochem/physiol Actions

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Physical form

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

biological source: egg white. Quality Level: 200. conjugate: agarose conjugate. form: aqueous glycerol suspension. matrix: cross-linked 6% beaded agarose. matrix activation: epichlorohydrin. matrix attachment: amino. matrix spacer: 15 atoms. storage temp.: −. 20Â°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.