Avidin protein, 25mg

A biotin-binding glycoprotein prepared from chicken egg white, Molecular weight=67kDa, pI = 10 Glycosylation and isoelectric point make avidin more soluble than streptavidin Significantly less expensive than recombinant streptavidin protein Based on strong binding with biotin, avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC) Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and Avidin binding with biotin makes it a useful in the diagnostic industry and has applications in purification of proteins Avidin is also used for the targeting of solid tumors and for applications in vitro such as protein purification and labeling