B834(DE3)PLYSS COMPETENT CELLS - NOVAGEN (C005B-232001)

General description

B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with ³⁵S-methionine and selenomethionine for crystallography (2).

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Genotype: F^-ompT hsdS_B(r_B^- m_B^-) gal dcm met (DE3) pLysS (Cam^R)





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B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression.

Features and Benefits

• Allows for ³⁵S-methionine or selenomethionine labeling for crystallography studies
• Deficient in lon and ompT proteases
• Guaranteed transformation efficiencies of >2x10⁶ cfu/μg
• Provided in a 0.2 mL volume; each vial can be used for 10 x 20μL transformations
• Prepared using an optimized chemical method

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlB834(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Other Notes

1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: Escherichia coli. manufacturer/tradename: Novagen^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: transformation efficiency: >. 2×10⁶ . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.

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