BACTIDENT AMINOPEPTIDASE

General description

BACTIDENT^™ Aminopeptidase test strips are diagnostic test strips used in microbiology to detect the activity of aminopeptidase enzymes in bacterial strains. Aminopeptidases are a group of enzymes that cleave amino acids from the amino terminus of polypeptides and proteins. The test strips contain L-alanine-4-nitroanilide as a substrate, which is split into 4-nitroaniline and the amino acid L-alanine in the presence of the enzyme alanine aminotransferase. The L-alanine aminopeptidase enzyme is localized in the cell envelope of bacteria, and the development of clear yellow coloration of 4-nitroaniline indicates the presence of the enzyme.

Application

BACTIDENT^™ Aminopeptidase test strips are useful for bioburden testing in the industry. The aminopeptidase test is a reliable method for determining the Gram behavior and differentiating Gram-positive from Gram-negative bacteria. These test strips are used for the detection of L-alanine aminopeptidase-producing microorganisms. The test is particularly useful in identifying Enterobacteriaceae and in differentiating between species of clinically important bacteria such as Streptococcus and Enterococcus.

Linkage

Replaces: 1.13301.0002

Analysis Note

Colour change to yellow
- Salmonella typhimurium ATCC 14028 (WDCM 00031):
+
- Aeromonas hydrophila ATCC 7966 (WDCM 00063): +
- Proteus rettgeri ATCC 29944: +
- Yersinia enterocolitica ATCC 9610 (WDCM 00038): +
- Bacillus cereus ATCC 11778 (WDCM 00001): -
- Staphylococcus aureus ATCC 25923 (WDCM 00034): -
- Enterococcus faecalis ATCC 11700: -

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany