Banana Multiplication Medium with Vitamins, Sucrose, Casein (C08-0444-738)

Banana Multiplication Medium has been developed for the in vitro multiplication of Musa species, family Musaceae. It is based on the Murashige and Skoog medium composition with certain alterations aiding towards suitability for Musa species. The formulation is a nutrient blend of inorganic salts, vitamins, carbohydrate, growth regulator and gelling agent. Banana Multiplication Medium provides all the essential macroelements and microelements. Potassium nitrate and ammonium nitrate serve as sources of nitrate and helps in organogenesis. This mixture of cation and anion is responsible for maintaining pH of the media. Potassium dihydrogen phosphate serves as a source of phosphate. Microelements like Boron, Manganese, Molybdenum, Iron, Copper, and Zinc enhance metabolism in the plants. Thiamine and Inositol act as enzymatic cofactors in universal pathways including glycolysis and TCA cycle along with primary and secondary metabolism in the plants. Casein hydrolysate is ideal for effective tissue proliferation as it serves as a source of amino acid. IAA helps to promote rooting. CleriGel™, a gellan gum is used as an alternative to agar. It offers several advantages over conventional agar as it sets a clear gel which assists easy observation of cultures and their possible contamination. Unlike agar, gel strength of CleriGel™ is unaffected over a wide range of pH and contains no contaminants like phenolic compounds that can be toxic to plant tissues. It solidifies uniformly and rapidly. The product is plant tissue culture tested but it is the sole responsibility of the user to ensure the suitability of the medium for individual species. Application: For the in Vitro Multiplication of Musa Species, Family Musaceae. It is Based on the Murashige and Skoog Medium Composition with Certain Alterations Aiding towards Suitability for Musa Species. The Formulation is a Nutrient Blend of inorganic Salts, Vitamins, Carbohydrate, Growth Regulator and Gelling Agent Appearance: White to off-white, homogenous, free flowing powder Solubility: 37.7 gms/litre soluble after boiling in distilled water Color and Clarity: Colorless to light yellow solution, clear gel is formed on cooling Gelling: Firm gel formed at pH: 5.75 ± 0.5