Cell Line Origin
Fish Brown Bullhead posterior trunk tissue
Cell Line Description
Derived from the trunk tissue (excluding fins) of a 2 year old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34°C. The optimal temperature range for cell proliferation is 25-30°C. BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.
Application
Virus studies: Infectious pancreatic necrotis virus and Channel catfish virus and channel catfish virus
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO2; 23°C. Note: The cells do not grow well on glass. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for a
Other Notes
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biological source: fish trunk. growth mode: Adherent. karyotype: 2n : 60. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 42294537
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87101201-1VL
