BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content even in the presence of detergents. This kit is based on the reduction of Cu2+ to Cu1+ by protein in alkaline solution; monovalent copper ions produced are detected in a concentration-dependent manner. Bicinchoninic acid (BCA) chelates with the reduced copper Cu1+ and forms a water-soluble purple reaction complex that exhibits a strong absorbance at 562 nm. Absorbance is linear over a wide range of protein concentrations between 25-2000 µg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard; the kit also includes Bovine Serum Albumin (BSA) at 1.5 mg/ml as a protein standard for a convenient preparation of protein concentration standard curves.
- UPC:
- 41122409
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- BCA2500
