BCL2 (SP66) Rabbit Monoclonal Antibody (C15-1301-315)

General description

BCL2 is a protein associated with apoptosis regulation produced by the bcl-2 gene, located on chromosome 14q32. BCL2 is comprised of an alpha (239 amino acids) and beta chain. BCL2 (and thus BCL2 alpha chain) is found in mitochondrial and nuclear membranes and in the cytosol rather than the cell surface. In normal lymphoid tissue, BCL2 (and BCL2 alpha) antibody reacts with small B-lymphocytes in the mantle zone and many cells within the T-cell areas. Anti-BCL2 alpha has shown consistent negative reaction on reactive germinal centers and positive staining of neoplastic follicles in follicular lymphoma. This difference in staining pattern is not due to down regulation or decreased BCL2 mRNA, but largely to a post-translational mechanism with resultant decrease in protein levels. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymphoid lesions. Anti-BCL2 has been used as an indicator of minimal residual disease in the bone marrow of follicular lymphoma patients when staining is strong and uniform.

Linkage

BCL2 Positive Control Slides, Product No. 226S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

biological source: rabbit. Quality Level: 100, 500. conjugate: unconjugated. antibody form: culture supernatant. antibody product type: primary antibodies. clone: SP66, monoclonal. description: For In Vitro Diagnostic Use in Select Regions (See Chart). form: buffered aqueous solution. species reactivity: human. packaging: vial of 0.1 . mL concentrate (226R-24), vial of 0.5 . mL concentrate (226R-25), bottle of 1.0 . mL predilute (226R-27), vial of 1.0 . mL concentrate (226R-26), bottle of 7.0 . mL predilute (226R-28). manufacturer/tradename: Cell Marque^™. . technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500. isotype: IgG1. control: tonsil. shipped in: wet ice. storage temp.: 2-8°C. visualization: cytoplasmic. Gene Information: human ... BCL2(596). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.