General description
The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP and MRP2 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional double knockout of the ABCG2 and ABCC2 (BCRP/MRP2) efflux transporters.
Application
The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Features and Benefits
The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight juntions which is necessary for efflux ratio analysis. Other benefits include:
- A functional double knockout of the BCRP gene and MRP2 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MDR1 substrate
- The vial format enables the BCRP and MRP2 knockout cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality
Legal Information
Shipping Information:
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- UPC:
- 12352200
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MTOX1006-1VL
- Temperature Control Device:
- Yes
