Cell Line Origin
Human Caucasian neuroblastoma
Cell Line Description
BE(2)-C is a clonal sub-line of SK-N-BE(2) (ECCAC catalogue no. 95011815) which was isolated from bone marrow of a 22-month-old male with disseminated neuroblastoma in 1972. They are reported to be multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine. The cells show a small, refractile morphology with short, neurite-like cell processes and tend to grow in aggregates.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Application
Chemotherapy development, chromosome studies, drug resistance, differentiation studies.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6
TPOX: 11
vWA: 18
Culture Medium
EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: human nerve. growth mode: Adherent. karyotype: Modal no. 44. morphology: Neuroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51131623
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95011817-1VL
