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be10-intermediaterat bdix foetal brain pre-ma (c15-1313-527)

BE10-INTERMEDIATERAT BDIX FOETAL BRAIN PRE-MA (C15-1313-527)

Sigma-Aldrich

Catalog No.
C15-1313-527
Manufacturer No.
93040119-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI
Price: $1,630.29
List Price: $1,811.43

Cell Line Origin Rat, BDIX, foetal brain, pre-malignant Cell Line Description Produced by passaging the latent period cell line BE10-Early (Sigma Catalogue number. 93040118) beyond passage 17.

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Cell Line Origin

Rat, BDIX, foetal brain, pre-malignant

Cell Line Description

Produced by passaging the latent period cell line BE10-Early (Sigma Catalogue number. 93040118) beyond passage 17. The cells possess elevated levels of tissue plasminogen activator activity, are not tumourigenic in BDIX rats and do not form colonies in agar. Do not be use beyond passage 40 as cell properties alter. The BE10 culture was originally derived 2 days after a single dose of ethylnitrosourea to pregnant rats. The control culture BE11 was derived 2 days after exposure to buffer.

Application

Study of changes in the latent period of carcinogenesis

Culture Medium

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: rat brain. growth mode: Adherent. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
51143705
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
93040119-1VL


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