beta-Amylase from sweet potato (C15-1289-752)

General description

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.

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Application

β-Amylase from sweet potato has been used:

• as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)
• as a standard for calibration curve preparation in size exclusion chromatography
• as a model protein for small-angle X-ray analysis (SAXs)

Biochem/physiol Actions

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.