beta-Glucuronidase, Helix pomatia (C15-1302-980)

General description

Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.

Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.

Biochem/physiol Actions

β-Glucuronidase, a member of the 2 glycosyl hydrolase family, is involved in the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase catalyzes the complete hydrolysis of urinary conjugates. β-Glucuronidase serves as a possible candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. β-glucuronidase-responsive albumin binding prodrugs might be an effective therapeutic practice for treating solid tumors.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will liberate 1.0 µg phenolphthalein from phenolphthalein glucuronide per h at 37°C, pH 5.0.

Physical form

A 65% saturated ammonium sulfate suspension.

Reconstitution

Following reconstitution, store in refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Shibasaki, H., et al. 2001. Steroids66, 795.
Graef, V., et al. 1977. Clin. Chem.23, 532.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: Helix pomatia. Quality Level: 100. form: liquid. manufacturer/tradename: Calbiochem^®. . storage condition: do not freeze. solubility: aqueous buffer: soluble. foreign activity: Aryl sulfatase ≤. 2%. shipped in: wet ice. storage temp.: 2-8°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H317 - H334. Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312. Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.