beta-Secretase Substrate IV, Fluorogenic - Calbiochem (C15-1305-816)

General description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported K_cat/k_m values (min^-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu^28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. k_cat/K_m values (min^-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu^28p-memapsin 2 are 0.044 and 0.040, respectively.

Biochem/physiol Actions

Cell permeable: no

Primary Target
β-secretase substrate

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 2005.22. Empirical Formula: C₉₁H₁₂₉N₂₅O₂₅S. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, protect from light. color: red. solubility: DMSO: 5 . mg/mL. fluorescence: λ. _ex . ~350 . nm, λ. _em . ~490 . nm. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.