Cell Line Origin
Rat ACI spleen x Mouse Sp2/0 Ag-14
Cell Line Description
The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.
Immunogen
Human erythropoietin
Application
Immunological studies, isolation of erythropoietin by immuno-affinity methods.
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Rat x Mouse Hybridoma. ImmunoDon: Rat AC1 spleen cells. Myeloma: Sp2/0 Ag-14. Reactivity: Anti-Erythropoietin, human. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51131506
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 95050413-1VL
- Temperature Control Device:
- Yes
