RNA Gel-shift Analysis Assays

Anti-DOT1L DOT1 like histone lysine methyltransferase, 25ul

General description The antibody detects endogenous level of total Fibrillarin protein. Immunogen Peptide sequence around aa.

General description This antibody detects histone H1 and nuclesomal core proteins histones H2a, H2b, H3, H4. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

NFkB gel shift assays are assembled in 20&microl reactions containing 0.28 pmoles NFkB oligo in 10mM Tris (pH 7.

RPB3 is one of 12 subunits that make up the complete RNA polymerase, Pol II, the primary RNA polymerase in cellular metabolism. RPB3 is part of the core element of Pol II complex.

General description This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

General description TRBP1 Antibody: The human trans-activation response (TAR) RNA binding protein 1 (TRBP1) was initially identified as a protein that binds to the HIV-1 TAR RNA and activates the long terminal repeat (LTR) expression in the

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

Anti-TRMT11 tRNA methyltransferase 11 homolog, 25ul UN 1687

General description TRMT12 (tRNA methyltransferase 12 homolog) gene is localized to human chromosome 8q24, and is a homolog of yeast gene coding for a tRNA methyltransferase. Immunogen Antibody was raised against a 19 amino acid peptide near the

TRMU codes for tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase that catalyzes the 2-thiolation of uridine. Studies have reported that TRMU mutations affect the deafness-associated mutations in 12S ribosomal RNA.

Bis-Tris is a zwitterionic buffer an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed