Biological Buffers

PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in

DescriptionDNA contaminants can be introduced into PCR through a number of reagents. To minimize the risk of contaminant DNA during PCR, we offer 10x MTP Taq buffer to be used with MTP Taq DNA Polymerase (Sigma product D7442). Each lot of MTP Taq

DescriptionFor routine PCR amplifications.

DescriptionFor routine PCR amplifications.

DescriptionFor routine PCR amplifications.

DescriptionFor routine PCR amplifications.

Description10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl2, each dNTP at 200 μM, primers defining an approximately 500 base pair region of λ DNA at 1.0μM

Description10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl2, each dNTP at 200 μM, primers defining an approximately 500 base pair region of λ DNA at 1.0μM

DescriptionRecommended for use with the SeqPlex DNA Amplification Kits.

DescriptionRecommended for use with the SeqPlex DNA Amplification Kits.

Description10× RedTaq PCR Reaction Buffer for use with Sigma′s RedTaq DNA Polymerase, product code D 4309.10x REDTaq®PCR Reaction Buffer is a polymerase chain reaction buffer for use with REDTaq®DNA Polymerase.

Description10× RedTaq PCR Reaction Buffer for use with Sigma′s RedTaq DNA Polymerase, product code D 4309.10x REDTaq®PCR Reaction Buffer is a polymerase chain reaction buffer for use with REDTaq®DNA Polymerase.