General description

BIOshell^™ 400 Ã… Protein C18 columns contain 3.4 μm advanced Fused-Core^® particles with 400 Ã… pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Ã… fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 Â°C, and within the pH range of 1 to 9.

Application

UHPLC Analysis of Proteins on BIOshell^™ A160 Peptide C18 2.7 μm; BIOshell^™ A400 Protein C18, 3.4 μm; and BIOshell^™ IgG 1000 Ã… C18, 2.7 μm

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Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

material: stainless steel hardware (column). Quality Level: 100. Agency: suitable for USP L1. description: Shell Thickness (0.2 μ. m), Solid Core (3.0 μ. m). product line: BIOshell^™.. feature: endcapped. analyte chemical class(es): proteins. manufacturer/tradename: BIOshell^™.. packaging: 1 ea of . extent of labeling: 1% carbon loading. parameter: 600 bar max. pressure, 90 Â°C max. temp.. technique(s): HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable. L Ã— I.D.: 10 . cm Ã— 4.6 . mm . surface area: ~15 . m²/g . matrix: spherical silica particle platform, superficially porous particle. matrix active group: C18 (octadecyl) phase. particle size: 3.4 . μ. m. pore size: 3.4 . μ. m particle size, 400 . Ã…. operating pH: 1-8. separation technique: reversed phase.