BioSpectrometerr Reference Filter Set

The Eppendorf BioSpectrometer® basic makes your work easier with the following features: simplest handling, guided software procedures, operation directly on the device, preprogrammed methods, automatic evaluation of measurement data and clear presentation of results. The Eppendorf BioSpectrometer basic is a small, very compact spectrophotometer that combines easy handling with highly reproducible analysis. It can measure and record UV/Vis spectral ranges as well as measure individual wavelengths from 200 nm to 830 nm. Its freely selectable wavelengths provide maximum flexibility for all current and future applications. In addition to preprogrammed methods for standard measurements, users can create and store their own methods. Optimized menu navigation guides through the individual methods in a step-by-step process. All required entries are visible, no essential data is omitted. A help box explains the operational sequences and is available in 5 different languages. Features Absorbance measurement for one or more wavelengths, recording of wavelength scans from 200 nm to 830 nm (increment: 1 nm) Automatic evaluation and storage of measurement data with clear presentation of results Spectral graph to display sample purity with automatic ratio calculation Preprogrammed applications for quick start Freely programmable applications with evaluation via factor, standard or standard series and freely programmable parameters Two-wavelength method with substraction or division evaluation Integrated application and results memory Data transfer via USB interface, Ethernet or via e-mail and direct printout of results Guided software process to minimize errors Integrated self-test and calibration history Applications Nucleic acid quantification Direct protein quantification (UV 280 nm) Microvolume measurement via µCuvette G1.0 for highly concentrated samples Bacteria growth measurement (OD 600) Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins) Free selectable wavelengths, e.g., 340 nm: assays using NADPH or NAPH, 405 nm: assays using para-nitrophenol, 420 nm: assays using ortho-nitrophenol, 490 nm: colorimetric fructose determination, 490 nm: cytotoxicity assay