General description
The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Application
The E. coli strain BL21 (DE3) has been used:
- to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
- BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
- to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.
Features and Benefits
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Components
- BL21(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
biological source: Escherichia coli. grade: for molecular biology. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: competent cell type: chemically competent
transformation efficiency: ≥. 1 x 107 . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3.
- UPC:
- 41105330
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CMC0014-20X40UL
